Medical laboratories are a critical component of quality health care and provide essential data for patient care and treatment, disease prevention and control, and public health policy development. I-TECH recognizes laboratory strengthening as a core component of its work. I-TECH’s Laboratory Systems Strengthening (LSS) team’s mission is to improve laboratory operations for optimal patient care and treatment, disease surveillance and response, biosecurity, and policy development. The LSS team fosters the development of sustainable laboratory systems and laboratory capacity in multiple countries throughout the world and comprises staff at the University of Washington (UW) in Seattle, as well as locally based staff. The team leverages partnerships within UW and with external collaborators globally, and engages directly with Ministries of Health and funders to accomplish mutual objectives.